Spectral Diagnostics of the Femoral Compact Bone Condition in Gangrene and Phlegmon
E.V. Timchenko1,2, D.G. Glubokov2, P.E. Timchenko1, I.V. Bazhutova2, L.T. Volova2, F.R. Bazhutov3;
1Samara University, Samara, Russia;
2Samara State Medical University, Samara, Russia;
3Lomonosov Moscow State University, Faculty of Chemistry, Moscow, Russia
Abstract
Lower extremity amputations are most commonly necessitated by vascular diseases and extensive purulent-necrotic phlegmon associated with diabetes, with over 50% of diabetic patients developing complications that lead to high amputations within 15–20 years. In such cases, the surgeon’s primary goal is to save the patient’s life and reduce the need for amputation. Optical diagnostic methods, particularly Raman spectroscopy, have shown promise in various biomedical applications. This study aimed to evaluate the potential of Raman spectroscopy to detect spectral differences in femoral compact bone tissue from patients with gangrene and phlegmon. Allogeneic femoral compact bone samples were obtained from donors of both sexes with established gangrene or phlegmon, as well as from conditionally healthy individuals without bone pathologies. Raman spectra were acquired in the 400–2200 cm⁻¹ range using a 784.7 nm laser excitation, with data processed in Wolfram Mathematica 12 through noise filtering, polynomial fluorescence subtraction, and chemometric analysis. Spectral changes associated with phlegmon were identified, reflecting alterations in the mineral–organic matrix and likely indicating secondary bone damage. In gangrene, a decrease in the intensity of Raman bands corresponding to phosphates, carbonates, and amides was observed; these changes were less pronounced than in phlegmon, suggesting an initial stage of bone destruction. These findings demonstrate the feasibility of Raman spectroscopy for assessing femoral compact bone condition in gangrene and phlegmon, which may ultimately assist in tailoring treatment strategies for these diseases.
Speaker
Bazhutov Filipp
Lomonosov Moscow State University, Faculty of Chemistry
Russia
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