Assessment of Peripheral Hemodynamics by Diffuse Reflectance Spectroscopy in the Visible and Near-Infrared Range in the Cuprizone-Induced Demyelination Model
Veronika Nikolaevna Volodina¹, Anastasia Andreevna Chervakova1, Alexander Vladimirovich Kolpakov2, Diana Yurievna Sokolova2, Petr Evgenievich Zaitsev1, Ulyana Aleksandrovna Apukhtina1, Tatiana Ivanovna Yaremenko1, Mikhail Sergeevich Krasnov1, Nikolai Nikolaevich Kuznetsov1,3, Viktoria Vyacheslavovna Zherdeva1;
1A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia
2Faculty of Biomedical Engineering, Department of Biomedical Technical Systems, Bauman Moscow State Technical University, Moscow, Russia
3FN 5 “Chemistry”, Bauman Moscow State Technical University, Moscow, Russia
Abstract
Abstract
The present study evaluates the feasibility of diffuse reflectance spectroscopy in the visible and near‑infrared range (530–1000 nm) for non‑invasive monitoring of peripheral hemodynamics and tissue oxygenation in the cuprizone‑induced demyelination model in C57BL/6 mice. The experimental group received a diet containing 0.4% cuprizone for 90 days, while the control group was fed a standard diet. Previously, a statistically significant prolongation of recovery time after inhalation anesthesia was observed in demyelinated animals (10 min vs. 2–5 min in controls), suggesting the presence of systemic hemodynamic or metabolic disturbances. Diffuse reflectance spectra were recorded from the tail vein surface and processed using the moving‑average method. Significant differences between the groups were revealed: cuprizone‑treated mice exhibited a decrease in reflected signal intensity in the 550–600 nm range, which may indicate changes in hemoglobin concentration or oxygenation in peripheral vessels. The authors suggest that the recorded spectral changes correlate with the clinically observed delayed emergence from anesthesia and may reflect both central and peripheral mechanisms of dysfunction. The obtained results demonstrate the potential of diffuse reflectance spectroscopy as an additional non‑invasive tool for assessing systemic hemodynamic disturbances in preclinical models of neurodegenerative diseases, particularly multiple sclerosis.
Speaker
Volodina Veronika
Federal Research Centre of Biotechnology of the Russian Academy of Sciences
Russia
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