Optical clearing alters the sensitivity of laser speckle contrast imaging to flow velocity
Surkov Y. I. 1,2, Serebryakova I.A. 1,2, Fashchevskiy A. P. 1,Timoshina P.A., 1,2, Genina E. A.1, Tuchin V. V. 1,2,3,4
1 Institute of Physics, Saratov State University, Saratov, Russia
2 Scientific Medical Center, Saratov State University, Saratov, Russia
3 Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russia
4 Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the RAS”, Saratov, Russia
Abstract
Optical clearing improves visibility but can bias laser speckle contrast imaging (LSCI). We combined OCT and PCA-LSCI in separate paired agarose phantoms during fluorescein diffusion. Speckle metrics changed at constant flow, and DAC sensitivity to velocity increased with clearing. Local background correction increased the velocity-associated share of variance from 39.8% to 56.3% and reduced the concentration-associated share from 41.2% to 15.4%. Quantitative flow monitoring therefore requires calibration for the local optical state.
Speaker
Surkov Yuriy
Saratov State University, Saratov, Russia
Russia
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