Photobleaching fluorescent microscopy lymph flow velocimetry in lymphatic vessels associated with deep cervical lymph node in mice
Alexander I. Dubrovsky1, Inna A. Blokhina2, Alexander V. Dmitrenko2, Ivan V. Fedosov1, Oxana V. Semyachkina-Glushkovskaya2; 1Institute of Physics, Saratov State University, Saratov, Russia; 2Department of Biology, Saratov State University, Saratov, Russia
Abstract
The lymphatic vessel network is yet to be fully studied and is of great interest to the researchers in the biophysics and biomedical fields. The lymphatic system function is associated with the brain clearance and liquid drainage functions. The aim of this work was to develop a method for direct in vivo quantitative measurements of lymph flow velocity in afferent and efferent lymphatic vessels of deep cervical lymph nodes (dcLNs) in laboratory mice and to compare the flow of lymph in mice with and without photomodulation of central nervous system. A photobleaching fluorescent microscopy system and software with graphics user interface for operating it were developed and utilized in the measurements of lymph flow velocity in cervical lymphatic vessels in mice with and without photomodulation. Lymph flow velocity was measured quantitatively via a post-processing algorithm applied to the in vivo photobleaching microscopy data. The lymph flow rate in cervical lymphatic vessels in mice with photomodulation of central nervous system was higher than in mice without photomodulation. Photobleaching fluorescent microscopy system was capable of direct lymph flow velocity measurements in cervical lymphatic vessels associated with dcLNs in live laboratory mice. Photomodulation was found to result in the stimulation of the lymph flow in cervical lymphatic vessels.
This work was supported by the Russian Science Foundation (Grant No. 23-75-30001).
Speaker
Dubrovsky Alexander Ilyich
Institute of Physics, Saratov State University
Russia
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