Mechanisms of Age Differences in the Effectiveness of Phototherapy of Glioblastoma
Anastasiia D. Kolabukhova1, Inna A. Blokhina1, Aleksander V. Dmitrenko1, Mohammad Reza Rashidian Vaziri2, Ameneh Sazgarnia3,4, Armin Imanparast3,4, Alexander Shiorkov5,6
Affiliations:
1.Department of Biology, Saratov State University, Astrakhanskaya 82, 410012 Saratov, Russia.
2.Department of Physics, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
3.Medical Physics Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
4. Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5.Institute of Biochemistry and Physiology of Plants and Microorganisms,
6.Saratov Scientific Centre of the Russian Academy of Sciences, Saratov, Russia
Abstract
Glioblastoma (GBM) is an aggressive brain tumor with poor survival, particularly in the elderly. Aging is a key factor in GBM incidence and treatment resistance. A major factor is the age-related decline in the function of the meningeal lymphatic vessels (MLVs), which play an important role in regulation of brain drainage and immune surveillance. Transcranial photobiomodulation (PBM) has been proposed as a non-invasive method to suppress GBM growth by stimulating the MLV functions. However, it was unknown whether PBM remains effective in aged subjects with pre-existing lymphatic dysfunction. This study investigated the role of MLVs and brain drainage in age-related differences in resistance to GBM and sensitivity to PBM.
The studies were performed on 6- and 24-month-old rats using a fluorescent GBM model. PBM (1050 nm, LED) was performed for 14 days. It was shown that 24-month-old rats had reduced MLV function, decreased brain drainage, and lower resistance to GBM. PBM significantly increased survival and reduced tumor volume in 6-month-old rats by improving MLV function, facilitating the traffic of protective CD8+ cells to the tumor, and reducing intracranial pressure and brain edema. In contrast, PBM was ineffective in 24-month-old rats due to the age-related decline in MLV functions. Thus, PBM is an effective method for stimulating brain drainage and immunity against GBM in early, but not late, ontogenesis.
The research was supported by the Russian Science Foundation (project No. 25-45-20004)
Speaker
Aleksander Dmitrenko
Department of Biology, Saratov State University, Astrakhanskaya 82, 410012 Saratov, Russia
Russia
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