Development of a Nailfold Capillaroscopy System and a Simple Lightsheet Mesoscopy System Based on the OpenSPIM Open Project

Abstract

Nailfold capillaroscopy is a simple and non-invasive method that allows observation of capillary loops along their entire length due to the anatomical features of this region. The method is widely used in rheumatology for the diagnosis of systemic scleroderma and Raynaud's phenomenon. In parallel, open platforms for microscopy of optically cleared samples specimens are being actively developed in the field of bioimaging, such as mesoSPIM — a specialized light-sheet microscopy system that provides high axial resolution.
Describe the design features and tuning procedure of the optical system obtained by creating a light sheet microscope for adaptation to mesoSPIM tasks.
An approach to system configuration is proposed, including the selection and calibration of components: the use of long working distance objectives, adjustment of the lens to control the position and thickness of the light sheet, as well as calibration of parameters for various wavelengths and magnifications. Particular attention is paid to the stationary light sheet formed by the Galilean telescope from a cylindrical and spherical lens.
It is demonstrated that the reassembled system allows obtaining images with a resolution of up to 1.5 µm laterally and 3.3 µm axially while maintaining compatibility with various optical clearing methods. A holder has been developed that enables scanning the object from any side, as well as setting the rotation angle of the sample for more detailed analysis of the object.
The development of the light sheet provides an accessible and powerful platform for high-speed, high-resolution imaging of large biological samples, opening up new possibilities for neurobiological and embryological research.

Speaker

Makhortov Mikhail Andreevich
Saratov National Research State University named after N.G. Chernyshevsky
Russia

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