Development of photodynamic therapy for cancer using nanoconjugates and indocyanine green
Maria Afonina 1 , Elina Genina 1 , Alla Bucharskaya 2,3 , Natalia Shushunova 3 , Vadim Genin 1 , Vladimir Bogatyrev 4 ,Vitaly Khanadeev 4 , A Simonenko 4 , Boris Khlebtsov 4 , Valery Tuchin 1,2,5,6
1 Optics and Biophotonics Department, Saratov State University, Russia
2 Science Medical Center, Saratov State University, Russia
3 Department of Pathological Anatomy, Saratov State Medical University named after V.I. Razumovsky, Russia
4 Institute of Biochemistry and Physiology of Plants and Microorganisms, FRC “Saratov Scientific Centre of the Russian Academy of Sciences” (IBPPM RAS), Russia
5 Institute of Precision Mechanics and Control, FRC “Saratov Scientific Centre of the Russian Academy of Sciences”, Russia
6 Laboratory of laser molecular imaging and machine learning, National Research Tomsk State University, Russia
Abstract
The pilot study aimed to develop a combined technology of laser plasmon photothermal
therapy (PPT) and photodynamic therapy (PDT) in rats with transplanted cholangiocarcinoma PC-1. The novel chitosan-pectin-PEG-AuNRs conjugates (Ch@P@PEG@Au) doped with ICG were tested. The maximum of the plasmon resonance of the conjugates was observed at the wavelength 780 nm. Thirty minutes after injections, the tumors were irradiated percutaneously with an 808 nm diode infrared laser at a power density of 2.3 W/cm 2 for 15 min. Animals were removed from the experiment 72 hours and 21 days after therapy. Morphological studies of the tumor were performed on sections stained with standard and immunohistochemical methods.Diffuse reflectance spectra of skin above the tumor were measured within the range of 420–2100 nm before and after irradiation. The work was supported by RSF grant 23-14-00287-P.
Speaker
Afonina Maria
Optics and Biophotonics Department, Saratov State University, Russia
Russia
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