Detection of Stromal Collagen Alterations in Oral Squamous Cell Carcinoma with Ex Vivo Polarized Light Microscopy
Vathsala Patil
Department of Oral Medicine and Radiology
Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India vathsala.mcods@manipal.edu
Adarsh Kudva
Department of Oral and Maxillofacial Surgery
Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India adarsh.kudva@manipal.edu
Nirmal Mazumder
Department of Biophysics
Manipal School of Life Sciences Manipal Academy of Higher Education, Manipal 576104, Karnataka, India nirmal.mazumder@manipal.edu
Gagan Raju Department of Biophysics
Manipal School of Life Sciences Manipal Academy of Higher Education, Manipal 576104, Karnataka, India gaganraju24@gmail.com
Albin Joseph
Department of Biophysics
Manipal School of Life Sciences Manipal Academy of Higher Education, Manipal 576104, Karnataka, India albinjoseph.rs@gmail.com
Chetana Chandrashekar
Department of Oral Pathology and Microbiology
Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India chetana.c@manipal.edu
Abstract
Abstract—This study investigated the changes in oral potentially malignant disorders and oral squamous cell carcinoma in relation to stromal collagen remodeling using polarized light microscopy and quantitative image texture analysis. Biopsy specimens from OPMDs and OSCC patients, as well as healthy controls, had their H and E-stained folds examined via polarized light microscopy to determine the changes in collagen birefringence and structural arrangement in each case. Quantification was achieved through the use of the gray-level features extracted from the gray-level co-occurrence matrix, including angular second momentum, homogeneity, energy, contrast, dissimilarity, and correlation. There was significant change observed in the arrangement of collagen fibers from OSMF to OSCC, where there was a reduction in angular second momentum from 0.41±0.06 to 0.19±0.04 (p < 0.001) and homogeneity from 0.72±0.05 to 0.46±0.07 (p < 0.001). Energy was also considerably decreased from 0.64 ± 0.08 to 0.33 ± 0.06, while contrast was considerably increased from (2.1 ± 0.4 to 4.8 ± 0.6). Similarly, the discriminative capability is confirmed using the receiver operating characteristic analysis, which yielded a high AUC value of 0.91 in ASM. This has validated the polarization-based GLCM analysis for providing objective biomarkers of altered tissue structures, which is advantageous in early detection of oral cancer.
Speaker
Dr Vathsala
Manipal College of Dental Sciences, Manipal Academy of Higher Education
India
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