Raman spectroscopy method for differentiating streptococcal strains grown on agar media
E.V. Timchenko1,2, A.V. Lyamin2, K.A. Kayumov2, I.V. Bazhutova2, D.G. Glubokov2, L.T. Volova2, P.E. Timchenko1, Yu.D. Ityaksov1, F.R. Bazhutov3
1Samara University, 443086, Russia, Samara, Moskovskoye Shosse 34
2Samara State Medical University, 443099, Russia, Samara, Chapaevskaya St. 89
3M.V. Lomonosov Moscow State University, Faculty of Chemistry, Moscow, Russia., Leninskie Gory St., 1, building 3.
Abstract
As a result of the conducted studies and linear discriminant analysis, spectral differences were identified between the studied streptococcal strains. These differences manifest themselves at Raman lines of ~484 cm-1 (glycogen), ~1280 cm-1 (Amide III and rocking vibrations of CH2 groups of the protein matrix), and ~1330 cm-1 (phospholipids). These differences are due to the structural and metabolic characteristics of the studied bacterial strains.
It was demonstrated that Raman spectroscopy can be successfully applied to differentiate streptococcal strains.
These results can be used as a basis for developing an analyzer for differentiating streptococcal strains.
Speaker
Yurii Ityaksov
Samara University
Russian Federation
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