Synergy of LIFT- produced Metal Nanoparticles and antibiotics against clinical bacterial strains
Alena A. Nastulyavichus1, Eteri R. Tolordava1, Evgenia V. Ulturgasheva1, Sofia P. Babina1,Sergey I. Kudryashov1; P.N. Lebedev Physical Institute of the Russian Academy of Sciences, Russia
Abstract
The growing resistance of pathogenic bacteria, including their biofilm forms, to traditional antibiotics requires the development of new combination approaches to effectively suppress infections. The synergistic effect of two classes of antibiotics with metal nanoparticles applied by laser-induced forward transfer was studied in an in-vitro model. It was found that combinations of antibiotics with Cu and Ag nanoparticles significantly enhance antibiofilm activity, with the most effective combinations being those with Cu nanoparticles, which reduced the number of viable microorganisms below the detection limit for all studied strains of S. aureus and P. aeruginosa. FTIR spectroscopy revealed that the combined treatment with nanoparticles with ciprofloxacin caused more pronounced changes in the regions of amide bands (1644–1651 and 1536 cm⁻¹), polysaccharides (970–1034 cm⁻¹), and phosphate groups (1219–1266 cm⁻¹) compared to treatment with nanoparticles alone, indicating synergistic damage to the cell wall, protein structures, and extracellular matrix of bacteria. Raman spectroscopy showed a redistribution of intensities in the region of 1300–1650 cm⁻¹ and a weakening of characteristic bacterial bands, with the strongest changes observed for combinations with Ag and Au nanoparticles, and the differences between P. aeruginosa and S. aureus reflecting the structural features of the cell membrane bacteria.
Speaker
Nastulyavichus Alena
P.N. Lebedev Physical Institute of the Russian Academy of Sciences
Russia
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