Raman Spectroscopy Method for Assessing the Quality of Buffer Solutions Used in Biotechnology Laboratories

Abstract

The quality of buffer solutions is one of the determining factors in obtaining reliable and reproducible results in biotechnological experiments. Optical methods have found widespread use for various biomedical applications. Raman spectroscopy can be used to assess the quality of buffer solutions used in biotechnology laboratories.
Objective: To examine the potential application of Raman spectroscopy to assess the quality of buffer solutions (Dulbecco and Hanks).
Three groups of buffer solution samples and one group of drinking water, used as a reference sample with a known high content of ionic and organic impurities, were studied: 1 - drinking water (GOST R 51232-98) (control sample); 2 - Dulbecco's DPBS solution (DPBS) without Ca²⁺ and Mg²⁺; 3 - deionized water for solution preparation manufactured by the Biotech Research Institute; 4 - Hanks' balanced salt solution (HBSS).
The experiments were performed using Raman spectroscopy using an experimental setup.
A Raman spectroscopy study revealed that Hanks' solution and Dulbecco's solution without Ca²⁺ and Mg²⁺ were prepared using highly deionized water, as evidenced by the characteristic Raman response in the 1660-1700 cm⁻¹ region and the results of PLS-DA analysis. Raman spectroscopy has been demonstrated to be an effective non-destructive tool for characterizing and quality control various types of buffer solutions used in biotechnology laboratories. The presented buffer solution samples meet the quality requirements for biotechnology research reagents and can be considered a viable alternative to imported analogs.

Speaker

Andrei A. Chernukhin
Samara University
Russia

Discussion

Ask question