Spectral Analysis of Allogeneic Coatings of Combined Biosynthetic Mesh Implants
Polina I. Pyataeva1, Pavel V. Shulepov2, Elena V. Timchenko1,2, Pavel E. Timchenko2, Nicolay A. Ryabov1,2, Larisa T. Volova1,2; 1Samara University, Samara, Russia; 2Samara State Medical University, Samara, Russia
Abstract
Synthetic materials remain the gold standard in hernia repair, yet their implantation inevitably provokes a foreign body response and subsequent chronic inflammation. To mitigate these adverse effects, the BioTech Research Institute of Samara State Medical University has developed an allogeneic combined biosynthetic implant comprising a polyvinylidene fluoride (PVDF) mesh coated with human tissue-derived material. This biocoating serves as a barrier between the synthetic scaffold and host tissues. Given that new medical devices require thorough product evaluation, quality assessment of such implants is of paramount importance.
Optical techniques are extensively applied in biomedical research. Among these, Raman and infrared spectroscopies are complementary vibrational methods that enable detailed characterization of chemical bonds and molecular structures. The present study aimed to evaluate the feasibility of using vibrational spectroscopy for quality control of allogeneic coatings on combined biosynthetic mesh implants.
The materials examined included uncoated PVDF implants, PVDF implants coated with a collagen-containing hydrogel, and the hydrogel itself, derived from human tendons. Coating quality was assessed via an experimental Raman setup and dedicated spectral processing software, with linear discriminant analysis (LDA) employed for in-depth spectral data interpretation.
IR spectroscopy was performed as an adjunct optical method using an FT‑801 Fourier spectrometer (Russia, SIMEX) equipped with an attenuated total reflection attachment. Raman spectroscopy combined with LDA revealed the spectral signatures of both the applied hydrogel and its pure form, demonstrating 93% spectral similarity between the two groups—indicating uniform hydrogel distribution over the mesh surface.
IR spectroscopy further confirmed that hydrogel application does not induce collagen denaturation and that collagen stabilization occurs on the implant surface.
Collectively, the integration of vibrational spectroscopy with linear discriminant analysis constitutes an effective non‑destructive strategy for quality control of biocoatings on biosynthetic mesh implants.
Speaker
Pyataeva Polina
Samara University
Russia
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