Detection of the anti-tuberculosis antibiotic linezolid using AgInS/ZnS quantum dots

Abstract

Tuberculosis is included in the list of ten socially significant diseases in the Russian Federation due to its infectious nature, latent development, long and complex treatment. Especially dangerous are mycobacterial strains resistant to the earliest developed anti-tuberculosis drugs. Linezolid (Lzd) is an effective oxazolidinone antibiotic widely used against multidrug-resistant tuberculosis. However, Lzd is myelotoxic, which leads to anemia and thrombocytopenia. Monitoring of drug concentration in patient biofluids can be the instrument for side effect prevention.
Quantum dots (QDs) are semiconductor nanocrystals, highly interesting for medicine, biochemistry, electrochemistry, photocatalysis and biophotonic. Their bright fluorescence is sensitive to the environment and its changes can serve as an analytical signal for the detection of various organic and inorganic substances. This report discussed the ability of ternary quantum dots AgInS/ZnS covered with thioglycolic acid to be the fluorescence source for Lzd detection.
First we examine the influence of the quantum dots size on the Lzd detection in water as the simplest model system. The size varied in the range of 4-7 and wavelength of fluorescence maximum in the range of 560-635 nm. It is shown that the lowest limit of detection was achieved with 7 nm QDs (λmax = 620 nm). The second part of report devoted to the Lzd detection in diluted human plasma, serum and plasma with proteins precipitate by organic solvents (acetonitrile, isopropanol and perchloric acid). Moreover, the influence of pH in the system is analyzed. Quantitative data such as limits of detection and Stern-Volmer constants are presented.

Speaker

Vera V. Rodionova
Saratov State University, Saratov, Russia
Russian Federation

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