Experimental evaluation of the functional properties of carbon-based coatings for titanium biomedical devices
Kristina D. Efremova1,2, Alexander Yu. Gerasimenko1,2; 1Institute of Biomedical Systems, National Research University “Moscow Institute of Electronic Technology,” Zelenograd, Moscow, Russia; 2Institute of Bionic Technologies and Engineering, I.M. Sechenov First Moscow State Medical University, Moscow, Russia
Abstract
An experimental method for determining the functional properties of coatings for titanium medical devices that interact with biological media is presented. A coating formulation based on multi-walled carbon nanotubes and type II collagen biopolymer was proposed to reduce thrombotic activity and improve surface performance under blood-contacting conditions. A microfluidic system was developed as a tool for in vitro testing of coatings under dynamic flow conditions with a controlled shear stress range of 50–150 Pa, which corresponds to physiologically relevant and pathophysiologically elevated flow regimes. An assessment of mechanical stability showed that during 3.5 hours of perfusion, the degree of coating degradation was 2.48 ± 0.19% at 50 Pa, 5.08 ± 0.67% at 100 Pa, and 7.63 ± 0.48% at 150 Pa, corresponding to the preservation of more than 92% of the initial thickness even at the maximum load. Analysis of thrombogenic properties based on bovine serum albumin adsorption demonstrated a decrease in adsorption intensity with increasing shear stress and also showed that the relative adhesion of albumin to carbon-based coatings is approximately 1.6 times lower compared with the titanium surface. These results indicate that collagen/multi-walled carbon nanotube coatings combine good flow resistance with reduced protein adsorption and limited protein corona formation. The proposed coatings are promising for surface modification of implantable biomedical devices operating under elevated blood flow shear stress.
Speaker
Efremova Kristina Dmitrievna
MIET
Russia
Discussion
Ask question
