Developing multimodal fiber‑optic probe and calibration materials for combined laser spectroscopy of intracranial tumors
Tatiana A. Savelieva1,2, Vadim Yu. Yunanov2, Igor D. Romanishkin1, Alexander V. Borodkin1, Kirill G. Linkov1, Anastasia V. Ryabova1,2,3, Svetlana V. Shugay4, Alexandra V. Kosyrkova4, Galina V. Pavlova4,5, Igor N. Pronin4, Victor B. Loschenov1,2; 1Prokhorov General Physics Institute of Russian Academy of Sciences, Moscow, Russia; 2 National Research Nuclear University MEPhI (Moscow Engineering Physics Institute), Moscow, Russia; 3RUDN University, Moscow, Russia; 4N.N. Burdenko National Medical Research Center of Neurosurgery, Moscow, Russia; 5Institute of Higher Nervous Activity and Neurophysiology of the Russian Academy of Sciences, Moscow, Russia
Abstract
Intraoperative navigation during the removal of intracranial tumors is of great practical importance, as the incidence of recurrence and postoperative complications depends on the accuracy with which tumorous tissue is demarcated from healthy tissue. One of the most promising approaches to addressing this challenge involves the use of laser optical spectroscopy methods, which offer rapid analysis and provide a wide range of tissue characteristics that distinguish tumors from normal tissue. In our project we developed method and device for multimodal laser spectroscopy of intracranial tumors, based on the integration of several optical-spectral techniques. We integrated 5‑ALA–induced fluorescence, diffuse reflectance, and Raman spectroscopy in a single fiber‑optic probe designed in the form of a neurosurgical aspirator, featuring special channels for optical fibers within the cannula wall. To calibrate each of the spectroscopic methods, optical phantoms were created containing the key tissue components that our method is designed to detect. Combined with Monte Carlo modeling and machine learning–based spectral analysis, the system provides quantitative, label‑free as well as fluorescence‑guided characterization of brain tumor tissue, advancing biophotonic technologies for neurosurgical oncology. This approach was tested at the cryopreservation laboratory of the Burdenko National Medical Research Center for Neurosurgery using fresh samples of tumors and adjacent tissues, with the aim of subsequently translating the technique to the intraoperative setting.
Speaker
Tatiana A. Savelieva
Prokhorov General Physics Institute of Russian Academy of Sciences
Russia
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