Assessment of Optical Parameters Changes in Biological Tissues via Diffuse Reflectance Spectroscopy Using Sapphire-Fiber Probe.

Abstract

Currently, integrating optical monitoring methods for biological tissues into clinical practice represents a highly relevant and promising direction. Measuring tissue optical parameters serves as an effective non-invasive diagnostic tool, enabling the assessment of physiological indicators such as oxygen saturation, perfusion, microcirculation, and chromophore concentration, as well as the differential diagnosis of pathologies, including tumors and diabetes complications.
This work presents a compact fiber-optic probe with a sapphire contact part designed for intraoperative clinical applications. Sapphire instruments are characterized by high hardness, thermal stability, corrosion resistance, biological and chemical inertness, and optical transparency in the visible range.
Diffuse reflectance spectroscopy was selected as the optical method, as it allows relatively accurate parameter measurements with simple hardware implementation and adaptability to various application conditions. The probe design incorporates three working channels with optical fibers connected to a halogen broadband source for illumination and to spectrometers for detection, spaced at distances of 1.3 and 2.6 mm.
This technique was tested on liquid tissue-mimicking phantoms. Fat emulsions at various concentrations were added to alter the scattering coefficient. Furthermore, to modify the absorption coefficient, phantoms were fabricated with dyes exhibiting absorption peaks at different wavelengths, alongside Agat-hemoglobin at varying concentrations.
Experimental studies confirmed the high sensitivity of the probe to optical property changes in the tissue-simulating phantoms, demonstrating its significant potential for modern medicine and biophotonics.

Speaker

Alina Platonova
Prokhorov General Physics Institute of the Russian Academy of Sciences
Russia

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