Application of infrared spectroscopy to the study of biopsies of patients with glial brain tumors
Vera E. Sitnikova1, Shokhzoda A. Tukhtaeva 2, Sergey V. Voloshin3,4, Dmitry A. Petrenko5, Liudmila V. Plotnikova6 1 ITMO University, Saint Petersburg, Russia; 2 Tashkent medical academy, Tashkent, Uzbekistan; 3 Kirov Military Medical Academy, Petersburg, Russia; 4 Leningrad Regional Clinical Hospital, Petersburg. Russia; 5 Saint-Petersburg Mining University, Petersburg, Russia; 6 St. Petersburg State University, St. Petersburg. Russia;
Abstract
The treatment of each pathology directly depends on the effectiveness of diagnostics. Brain tumors (BT) are often detected only at late stages, since most existing instrumental diagnostic methods are mainly used after the manifestation of signs of the disease, when the tumors reach clinically significant sizes. Despite significant advances in neurosurgery and oncology, glial brain tumors continue to be an acute problem in modern healthcare. The relevance of developing new diagnostic methods is due to the prevalence and social significance of glial tumors, the great need to develop minimally invasive methods of early diagnosis to improve the overall duration and quality of life of such patients, as well as express diagnostic methods to reduce the time of diagnosis using traditional methods.
The work assessed the possibility of using infrared spectroscopy of attenuated total internal reflection (FTIR ATR) as a method for early diagnosis of glial tumors on biopsies of patients (blood serum) in comparison with control samples; as well as an express method of classification within a given type of tumor (brain tissue).
In this work, serum from patients with various brain tumors and healthy controls was examined. Glioblastoma, astrocytoma, oligodendroglioma and brain metastases were successfully separated from control samples using PLS and PCA-LDA methods with a sensitivity and specificity of over 92%. Analysis of the corresponding amide I bands showed minor variations in the levels of various protein secondary structures within each tumor type.
Speaker
Sitnikova Vera
ITMO University
Russia
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