Raman spectroscopy for assessing the composition of water used in biotechnical laboratories
Pavel E. Timchenko1, Artem D. Volov2, Elena V. Timchenko1,2, Larisa T. Volova3, Andrey A. Chernukhin1; 1Samara State Aerospace University, Samara, Russia; 2Samara State Technical University, Samara, Russia; Samara State Medical University, Samara, Russia
Abstract
Water quality plays an important role in experimental studies in biotechnological laboratories. Neglect of water quality in laboratory applications can lead to unsatisfactory results of the experiments.
Raman spectroscopy can be used to assess the quality of water used in biotechnical laboratories.
The aim of the work is to apply Raman spectroscopy to assess the composition of water used in biotechnical laboratories
The objects of the study were: 1- BiotechAqualab AL-2 EDI - deionized water, obtained on the Aqualab AL-2 EDI device; 2- Biotech membrane DME-2B, 2- OPTIMA - deionized water, obtained on the DME-2B OPTIMA device, 3- Ultrapure Atlant - deionized water, third-party manufacturer, purity 99.999% and 4 - tap water.
The processing of Raman spectra was carried out in the program "Raman spectroscopy toolv. 1.0.9.
Additionally, a chemical analysis of the studied samples was carried out.
The spectral analysis of the samples showed that the spectra of deionized water obtained with the Aqualab AL-2 EDI device are practically free of lines ~1560 (n(CN) and d(NH) amide II (protein assignment)) and ~1660 cm–1 (Amide I), unlike the other two groups studied, which may indicate that it is the most purified from organic compounds. The results of the chemical studies show that the water obtained from each of the devices — Aqualab AL-2 EDI and DME-2/B OPTIMA, as well as purchased deionized water from a third-party manufacturer, presented under the brand name “ultra-clean” (OOO “Atlant”), have approximately the same chemical composition.
Despite the presence of microbiological contamination in the analyzed samples, the water obtained using the Aqualab AL-2 EDI device is the most suitable for use in biotechnological laboratories due to the minimal level of contamination.
Speaker
Andrey . A. Chernukhin
Samara State University
Russia
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