Raman spectroscopy for evaluation of decellularized grafts in burn injury treatment

Abstract

One of the main tasks of treating burn victims throughout the history of combustiology is the timeliness and completeness of restoration of skin areas lost as a result of burn injury. In recent years, biogenic materials have been widely used in various fields of medicine to replace tissue defects. Currently, optical research methods have found wide application to assess the composition of biomaterials
As a result of the conducted studies, spectral differences were established between the native group of the studied objects and the group of objects processed with ultrasound, which are manifested in the following Raman lines ~1650 cm-1 (Amid I), ~1240 cm-1 (Amid III), ~1100–375 cm-1 (Several bands of moderate intensity, belonging to anide III and other groups (proteins)), ~600-800 cm-1 (Nucleotide conformation), ~481 cm-1 (DNA), ~447 cm-1 (Ring torsion of phenyl).
These changes are due to a decrease in the antigenicity of the samples after ultrasound treatment, as well as a change in amide structures due to anionesis.
It was also established that ultrasound treatment, according to the patented LIOPLAST method, ensures the preservation of the structure of the studied biomaterials.
Detailed mathematical processing using statistical methods of analysis and ROC analysis showed high efficiency of separation between the studied groups.
It is shown that using the method of Raman spectroscopy it is possible to carry out express analysis of the composition of decellularized biomaterials during their manufacture with specified properties for specific tasks.

Speaker

Yurii Ityaksov
Samara National Research University named after Academician S.P. Korolev
Russian Federation

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