Optical system for pH monitoring in microfluidic chips
Ivan Khaustov1, Andrey Bogomolov1,2, Eugene Bazarnov2, Vladislav Korneev2; 1 High School of Economics University, Moscow; 2 Samara State Technical University, Samara, Russia.
Abstract
Microfluidic devices (chips) are miniature bioreactors that simulate the functioning of various human organs, such as the liver, lungs, and skin, under conditions close to in vivo. In a functioning chip, cells are cultivated in a nutrient medium, which is accompanied by various chemical processes that require in-line monitoring. One of the most important control parameters is the pH of the medium, which is associated with cell metabolism. Therefore, an acid-base indicator is usually added to the medium and a visual color change is observed. For more accurate control, a non-invasive (without sampling) numerical method for pH assessment is required. Traditional electrochemical and optical spectral methods are poorly suited for many reasons: bulkiness, requirements for cultivation conditions, small volumes, etc. Therefore, our project aims to develop an OMS for determining pH directly in the microfluidic circuit of the chip. We assume that a sensor based on an RGB LED with an extended color gamut (for example, 26 shades) will be able to determine pH with high accuracy.
Speaker
Ivan Khaustov
High School of Economics University, Moscow
Russia
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