The effect of the dose of nanocomplexes administered on morphological changes in the internal organs of white laboratory mice
Artyom M. Mylnikov1, Nikita A. Navolokin1, Irina Yu. Yanina2; 1Saratov State Medical University, Saratov, Russia; 2Saratov State University, Saratov, Russia;
Abstract
Functionalized upconversion nanoparticles are regarded as a promising avenue for advancing photodynamic therapy, as they can not only enhance therapeutic efficacy but also broaden diagnostic capabilities. Covalent anchoring of a photosensitizer to the particle shell requires additional surface modification. Serum albumin is frequently used as a protective and functional coating: it is biocompatible with many cell types over a wide concentration range, is readily internalized by cells, is essentially non-immunogenic, and enables prolonged circulation in the bloodstream. In the clinical development of such agents, a key step is determining the optimal (most effective) dose. Following intravenous administration of the particles to white laboratory mice, signs of circulatory disturbances were observed in internal organs at all three dosage levels. Mild congestion and moderate edema were detected in the myocardium at all doses and time points. In the lungs, pronounced congestion was recorded one week after administration at doses 2 and 3, and at doses 1 and 3 one day after administration. In the kidneys, findings included pronounced congestion, hemorrhage in the renal stroma (at dose 3 one week post-administration), and moderate congestion in the liver, along with pronounced congestion in some cases. In the liver, at doses 1 and 2, and in the kidneys at dose 2 one week post-administration, the severity of cellular damage increased. The most prominent signs of epithelial injury in the convoluted tubules were observed at dose 3 (both one day and one week after administration), while in the liver such pronounced damage was evident one week after administration of dose 3.
The study was supported by a grant Russian Science Foundation No. 25-22-00144,
https://rscf.ru/project/25-22-00144/
Speaker
Муlnikov Artyom
Assistant department of Pathological Anatomy, SSMU named by V.I. Razumovsky
Russia
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