Optical clearing of skin using enhancers of tissue permeability in a wide spectral range
Daria K. Tuchina,1 Valery V. Tuchin1,2,3;
1Institute of Physics and Science Medical Center, Saratov State University, Saratov, Russia
2Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, Tomsk, Russia
3Institute of Precision Mechanics and Control, FRS “Saratov Scientific Centre of the RAS”, Saratov, Russia
Abstract
Measurements of diffuse reflection and total transmission spectra of rat skin samples ex vivo were performed in the spectral range of 350-1500 nm before and after 30-minute immersion in an aqueous 70% glycerol solution with and without the addition of 10% dimethyl sulfoxide (DMSO), as well as with ultrasound (phonophoresis) impact on the samples. The optical parameters of the samples were recorded on UV-3600 spectrophotometer with an integrating sphere (Shimadzu, Japan). The measured skin spectra were used to calculate the spectral dependences of the transport scattering coefficient and absorption coefficient of the samples.
The values of the transport scattering coefficient and absorption coefficient of rat skin ex vivo decreased after optical clearing in the studied spectral range in each group of samples. The transport scattering coefficient of samples decreased by an average of 1.5 times, and the absorption coefficient – by 1.7 times, at immersing the samples in a aqueous 70% glycerol solution. The decrease in the transport scattering coefficient and absorption coefficient was more significant (2.2 and 4.4 times, respectively) at adding permeability enhancers to the optical clearing by glycerol solution. A tendency toward an increase in the efficiency of optical clearing of the skin was observed at adding DMSO and ultrasound to the glycerol solution.
This work was supported by the Russian Science Foundation grant No. 23-14-00287.
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Daria K. Tuchina
Saratov State University
Russia
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