Fluorescence imaging method for diagnosis of oral mucosa pathologies

Abstract

The autofluorescence intensity of oral mucosa tissues can serve as a diagnostic criterion for detecting the pathologies due to high correlation between changes in the endogenous fluorophore’s concentration, such as FAD, and development of lesions. The work is aimed to assess the difference in the level of FAD autofluorescence of different cell types in the blue-green spectrum for development of a fluorescence imaging device.
Healthy human skin fibroblasts, B16 and В16F10 melanoma cells were selected as the study object. Autofluorescence was monitored by the Zeiss LSM 900 laser scanning confocal microscope (excitation wavelength – 488 nm, autofluorescence registration – 505-550 nm).
Results showed that the autofluorescence intensity of healthy skin fibroblasts exceeds the autofluorescence intensity of melanoma cells by 4-5 times with a statistically significant difference between experimental groups (*p <0.05 according to the Mann-Whitney criterion). Previous studies showed that a high-intensity autofluorescence leads to cell death after 24 hours. Thus, the high level of FAD autofluorescence can serve as a marker of metabolic disorders leading to cell death, while an ultra-low level of autofluorescence can be a marker of cancer.
Thus, the use of fluorescence imaging to assess the oral mucosa conditions seems to be perspective for detection presence of pathology. The use of image registration channel in developing device of fluorescence imaging will significantly reduce the false negative diagnostic result compared to used technical solutions in the practice of a dentist.

The study was funded by Russian Science Foundation according to the research project № 24-75-00144, https://rscf.ru/project/24-75-00144/.

Speaker

Ekaterina Bryanskaya
Research and Development Center of Biomedical Photonics, Orel State University
Russia

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