The photodynamic therapy of transplanted colorectal carcinoma in normal and immunodeficient mice
Alla B.Bucharskaya1,2, Nikita N. Navolokin1,2, Vadim D. Genin, Galina N. Maslyakova, Natalya A. Shushunova1,2, Olga I. Guslyakova2, Olga A. Inozemtseva2, Elina A. Genina2, Valey V.Tuchin2,3; 1Saratov State Medical University, Saratov, Russia; 2Saratov State University, Saratov, Russia; 3Institute of Precision Mechanics and Control RAS, Saratov, Russia
Abstract
The aim of the study was to evaluate the effectiveness of photodynamic therapy (PDT) in normal and immunodeficient mice with transplanted colorectal carcinoma CT-26.
Balb/c and immunodeficient nu/j mice were injected intramuscularly with 20 µl of CT-26 colorectal cancer cell suspension. When the tumor volume reached 500 mm3, they were randomly divided into 2 groups - with photodynamic therapy and without any treatment.
Indocyanine green was used for PDT, which was diluted in polyethylene glycol in a ratio of 1:100 and administered intravenously to mice at a dose of 2 mg/kg. One hour after intravenous administration, the tumors were irradiated with a percutaneous diode infrared laser with a wavelength of 808 nm at a power density of 2.3 W/cm2 for 10 minutes. The animals were removed and tumor tissues were collected for histological examination 72 hours and 21 days after therapy. After 72 hours, necrotic changes were observed in the tumors, necrosis fields occupied up to 70-80% of the area, 21 days after PDT, inhibition of tumor growth was noted, which was more pronounced in Balb/c mice than in immunodeficient mice, this indicates the importance of evaluate the impact of PDT on immune system in cancer treatment.
Speaker
Alla Bucharskaya
Saratov State Medical University
Russia
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