Endogenous fluorophores of sperm from farm animals and fish
Plavskii V.Y., Ananich T.S., Sobchuk A.N., Mikulich A.V., Tretyakova A.I., Plavskaya L.G., Leusenka I.A., Yakimchuk S.V., Institute of Physics NAS of Belarus, Minsk, Belarus
Abstract
Interest in the study of endogenous fluorophores present in animal sperm is due to their ability to generate reactive oxygen species, which at low concentrations can act as signaling molecules determining the effects of photobiomodulation.
In this work, we investigated the absorption, spectral-fluorescence characteristics and fluorescence decay kinetics of sperm components of farm animals (boars and goats), as well as valuable fish species (sturgeon).
To identify the main fluorophores, both native sperm and extracts of fluorescent compounds from sperm with organic solvents and hydrochloric acid were used. The following fluorescent components were identified in the sperm of the above animals and fish: coenzymes NADH and NADPH, flavins FAD and FMN, non-metal porphyrins (primarily coproporphyrin and uroporphyrin); the presence of lipofuscin-like pigments is also possible.
These compounds, when photoexcited, generate active forms of oxygen (primarily singlet oxygen), which at low concentrations are capable of changing the redox state of spermatozoa and their functional fertilizing characteristics. This approach allows the use of light to increase the efficiency of artificial insemination of animals.
Speaker
Plavskii V.Y.
Institute of Physics NAS of Belarus, Minsk, Belarus
Belarus
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